Dark Neurons of the Sensorimotor Cortex of White Rats after Acute Incomplete Ischemia in Terms of Artifacts Fixation and Neuroglial Relationships

The aimof research was to study morphofunctional signs of the sensorimotor "dark neurons" formation in cerebrum sexually mature white rats under normal conditions and 40 minutesafter common carotid arteries (CCA) occlusion. Material methods.Acute ischemia simulated Wistar by a 40-min CCA brain fixed immersion perfusion methods. A comparative morphometric assessment cyto-and gliocytoarchitectonics neocortex carried out (n = 6), 1 3 (n= 7 days 6) after Nissl staining, hematoxylin eosin immunohistochemical typing NSE, MAP-2, HSP-70, p38, CASP3, GFAP AIF1 were applied. numerical density normochromic hyperchromic (dark) pyramidal neurons, oligodendrocytes, microgliocytes, relative area edema –swelling zones determined. Statistical hypotheses tested nonparametric methods using Statistica 8.0 software. Results.The method fixation significantly influenced content artifact dark neurons,which are similar morphology tinctorial properties degeneratively altered neurons. appearance reversibly irreversibly neurons cortex occlusion accompanied an increase (control –5.4%, 1st day –17.6%). maximum (53%) found layer V ischemia. According findings specific proteins most retained, ensuring their restoration neural network functioning. total number decreased 26.4% (p =0.001) III, 18.5% (Mann –Whitney U Test; p 0.01) intervention. revealed changes diffuse focal character. In accumulation, astrocytes, microgliocytes oligodendrocytes increased. neuro-astroglial index –1.62) increased 2.72 peak microgliocyte s noted day, acute incomplete p0.001). Conclusion.After 40-minute occlusion, detected layers III with underlying hydropic dystrophy; cytomorphological pro perties indicated dynamics vivo degenerative changes. Restoration satellite astrocytes microgliocytes. considered as one variants reversible response moderate ischemic damage.